N-Propargyl-L-arginine Is a Potent Inhibitor and Slow Substrate of Nitric Oxide Synthase, Walter Fast, Marc Levsky, Michael A. Marietta, and Richard B. Silverman, Department of Biochemistry, Molecular Biology, and Cell Biology and Department of Chemistry, Northwestern University, Evanston, IL 60208; and Interdepartmental Program in Medicinal Chemistry, College of Pharmacy and Department of Biological Chemistry, School of Medicine, University of Michigan, Ann Arbor, MI 48109.
T7 DNA Helicase Has Three Non-catalytic Nucleotide Binding Sites, A Possible General Feature for Hexameric Helicases, Kristen C. Moore, Manju M. Hingorani, M. Todd Washington, and Smita S. Patel, Department of Biochemistry, Ohio State University, Columbus, OH 43210.
T7 DNA Helicase: Mechanism of Nucleotide Triphosphate Hydrolysis and Similarities to the F1-ATPase, M. Todd Washington, Manju M. Hingorani, Kristen C. Moore, and Smita S. Patel, Department of Biochemistry, Ohio State University, Columbus, OH 43210.
Towards the Discovery of Antiparasitic Drugs: Mechanism Based Design and Syntheses of Inhibitors for Glutathionylspermidine Synthetase, Shoujun Chen, David S. Kwon, Chun-Hung Lin, William P. Malachowski, Christopher T. Walsh, and James K. Coward, Interdepartmental Program in Medicinal Chemistry, College of Pharmacy and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055; and Department of Biological Chemistry and Molecular Pharmacology, Harvard University Medical School, Boston, MA 02115.
Overexpression of S-Adenosyl-L-methionine Hydrolase, David L. Howe and Ronald W. Woodard, Interdepartmental Program in Medicinal Chemistry, University of Michigan, Ann Arbor, MI 48109-1065.
Further Studies of KDO 8-P Synthase: 3-Bromopyruvate Inactivation Studies and Further Site-Directed Mutagenesis, Mayur A. Patel, Christopher Adis, and Ronald W. Woodard, Interdepartmental Program in Medicinal Chemistry, University of Michigan, Ann Arbor, MI 48109-1065.
Probing the Active Site of Aspartate Aminotransferase Using NMR Methods, Emilia T. Mollova, David E. Metzler, and Agustin Kintanar, Department of Biochemistry and Biophysics, Iowa State University, Ames, IA 50011.
Preliminary Studies of the Cyclohydrolase of Histidine Biosynthesis, Robert L. D’Ordine, Thomas J. Klem, and V. Jo Davisson, Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-1333.
Definition of the Substrate Specificity of the ‘‘Sensing’’ Xylanase of Streptomyces Cyaneus Using Xylooligosaccharide and Cellooligosaccharide Glycosides of 3,4-Dinitrophenol, Yongxin Zhao, Calvin J. Chany, II, Paul F.G. Sims, and Michael L. Sinnott, Department of Chemistry, University of Illinois at Chicago, Chicago, IL, 60607-7061; and Departments of Biochemistry and Applied Molecular Biology, and Paper Science, UMIST, POB 88, Sackville Street, Manchester, M60 1QD, UK.
Investigation of the Active Site of Aspartokinase III from Escherichia coli, Yen-Fang Keng and Ronald E. Viola, Department of Chemistry, University of Akron, Akron, Ohio 44325-3601.
Mechanism of Hydrolysis of -Lactam Antibiotics, Devkumar Mustafi, Mona M. Knock, and Marvin W. Makinen, Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637.
Structural and Mechanistic Studies of E. coli Guanosine Monophosphate Synthetase, Michael L. Deras and V. Jo Davisson, Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-1333.
The Entatic State of Metal Ions: Confirmation of Apometalloenzyme Formation, James M. Willard and Ralph A. Gardner-Chavis, Cleveland State University, Cleveland, OH 44115.
Facilitated Diffusion of Ribonuclease A, Bradley R. Kelemen and Ronald T. Raines, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706-1569.
Protein–RNA Interactions: Importance of Coulombic Forces, Barbra M. Templer, Jeung-Hoi Ha, and Ronald T. Raines, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706-1569.
Recognition of Selected Azoles as Degenerate Bases by Thermostable DNA Polymerases, Geoffrey C. Hoops, Natasha Paul, Peiming Zhang, W. Travis Johnson, Donald E. Bergstrom, and V. Jo Davisson, Department of Medicinal Chemistry and Pharmacology, Purdue University, West Lafayette, IN 47907-1333.
Transient State and Equilibrium Studies of T7 RNA Polymerase Binding to T7 Promoters, Amarendra Kumar, Yiping Jia, and Smita S. Patel, Department of Biochemistry, Ohio State University, Columbus, OH 43210.
Studies on the Biosynthesis of Yersiniose in Yersinia Pseudotuberculosis Strain VI, Zhihong Guo and Hung-wen Liu, Department of Chemistry, University of Minnesota, Minneapolis, MN 55455.
A Novel Microbial Resistance Mechanism: Redox Chemistry in Mitomycin Resistance, David A. Johnson, Paul R. August, Hung-wen Liu, and David H. Sherman, Department of Chemistry, University of Minnesota, Minneapolis, MN 55455; and Department of Microbiology and Biological Process Technology Institute, University of Minnesota, St Paul, MN 55108.
Does CDP-Tyvelose Epimerase Utilize a Novel Mechanism? The Exploration Begins, Tina Hallis and Hung-wen Liu, Department of Chemistry, University of Minnesota, Minneapolis, MN 55455.
En Route to an Understanding of the Biosynthesis of dTDP-L -Rhamnose: A Quaint Characteristic of the Reductase, rfbD, Nanette Loida S. Que, Yenyoung Lei, and Hung-wen Liu, Department of Chemistry, University of Minnesota, Minneapolis,MN 55455.
Synthesis of -Fluoro-S-adenosylmethionine: A Potential Affinity Label of 1-Amino-cyclopropane-1-carboxylate Synthase, Caroline Leriche and Hung-wen Liu, Department of Chemistry, University of Minnesota, Minneapolis, MN 55455.
Biosynthesis of Streptomycin: Initial Studies of TDP-Dihydrostreptose Synthase p(strL) Involved in a Novel Ring Contraction Reaction, Xuemei M. H. Chen, Zhihong Guo, and Hung-wen Liu, Department of Chemistry, University of Minnesota, Minneapolis, MN 55455.
Protein Engineering of the Non-phosphorylatable Pseudomonas Mevalonii HMG-CoA Reductase: Construction of Mutant Enzymes Regulated by Reversible Phosphorylation, Jon A. Friesen and Victor W. Rodwell, Department of Biochemistry, Purdue University, West Lafayette, IN 47907-1153.
HMG-CoA Reductase from the Thermophilic Archaeon Sulfolobus Solfataricus, D. A. Bochar, J. R. Brown, W. F. Doolittle, H-P. Klenk, W. Lam, M. E. Schenk, and V. W. Rodwell, Departments of Biochemistry at Purdue University, West Lafayette, IN 47907-1153; and Dalhousie University, Halifax, NS, B3H 4H7 Canada.
Identification of the True Product of Urate Oxidase, Kalju Kahn, Peter Serfozo, and Peter A. Tipton, Department of Biochemistry, University of Missouri-Columbia, MO 65211.
Characterization of the Active Site Structure of L-Aspartase from Escherichia coli, Maithri Jayasekera and Ronald E. Viola, Department of Chemistry, University of Akron, Akron, OH 44325-3601.
Design of Artificial Enzymes Using Protein Cavities: Application to the Enantioselective Synthesis of Amino Acids, Ron Davies, Hao Kuang, Matthew Brown, and Mark D. Distefano, Department of Chemistry, University of Minnesota, Minneapolis, MN 55455.
Analogs of Farnesyl Pyrophosphate Containing Benzophate Containing Benzophenones and Iazotrifluoropropionates: Synthesis and Photoaffinity Labeling Experiments with Yeast Protein Farnesyltransferase, Tammy C. Turek, Igor Gaon, Rebecca Edelstein, Valerie Weller, and Mark D. Distefano, Department of Chemistry, University of Minnesota, Minneapolis, MN 55455.
The Effects of HIV-1 Nucleocapsid Protein on DNA Strand Exchange, Wendy R. Davis, Sam Gabbara, and James A. Peliska, Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109.
Ascaris Suum Phosphoenolpyruvate Carboxykinase; Expression and Active Site Studies, Sandra Rios and Thomas Nowak, Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556.
Solvent Isotope Effects on the Formation of Alpha Helices, Jose O. Nazario, Eric W. Griffith, and Vernon E. Anderson, Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106-4935.
HIV-1 Reverse Transcriptase Mutants with Increased Fidelity, Karen E. Macek, Wendy R. Davis, and James A. Peliska, Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109.
Purification and Characterization of Human Recombinant Lecithin: Cholesterol Acyltransferase, Lihua Jin, Yong-Phil Lee, and Ana Jonas, Department of Biochemistry, University of Illinois at Champaign-Urbana, Urbana, IL 61801.
Pea (Pisum sativum) Chloroplastic Glyceraldehyde-3-phosphate Dehydrogenase Has Uracil DNA Glycosylase Activity, Xingwu Wang and Louise E. Anderson, Department of Biological Sciences, University of Illinois at Chicago, Chicago,
IL 60607.
Effect of an Arginine 374 to Lysine Mutation in Yeast Enolase, M. G. Buening and T. Nowak, Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556.
Development of a General Method to Select and Characterize Crosslinked Peptides, Devayani Eswaran and Vernon Anderson, Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106.
Probing Metal Ion Catalysis in Phosphoryl Transfer Reactions, J. F. Curley, C. M. Joyce, and J. A. Piccirilli, Departments of Chemistry and Biochemistry and Molecular Biology and the Howard Hughes Medical Institute, University of Chicago, Chicago, IL 60637; and Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520.
Use of a Synthetic DNA Module to Generate Mutations in the Activation Domain of Cobalamin-Dependent Methionine Synthase, Kerry Fluhr, Jeffrey D. Scholten,and Rowena G. Matthews, Department of Biological Chemistry and Biophysics Research Division, University of Michigan, Ann Arbor, MI 48109; and Parke–Davis Research Division, Warner-Lambert, Ann Arbor, MI 48105.
Mechanism of Inactivation of Cysteine Proteases by 3H-1,2 Benzodithiol-3-one-1-oxide, Brian C. Ganley, Leonid Breido, and Kent S. Gates, Departments of Chemistry and Biochemistry, University of Missouri-Columbia, Columbia, MO 65211.
Kinetic Analysis of the Catalytic Domain of Human Cdc25B, Elizabeth B. Gottlin, Xu Xu, David M. Epstein, Shannon P. Burke, Jens W. Eckstein, David P. Ballou, and Jack E. Dixon, Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109; and Mitotix Inc., Cambridge, MA 02139.
Chemical Modulation: An Extension of Site-Directed Mutagenesis, S. Kirk Wright and Ronald E. Viola, Department of Chemistry, University of Akron, Akron, OH 44325-3601.
Structure of Hexadienoyl-CoA Bound to Enoyl-CoA Hydratase Based on NOE and Homology Modeling, Wen-Jin Wu, Daniel P. Raleigh, Peter J. Tonge, and Vernon E. Anderson, Department of Chemistry, SUNY at Stony Brook and Case Western Reserve University, Cleveland, OH 44106-4935.
Synthesis of Molecular Probes for Determination of Biological Target of Antiangiogenic Drug AGM-1470, Nanaji K. Bhamare, Robert J. Kubiak, and Karol S. Bruzik, Department of Medicinal Chemistry and Pharmacognosy, University of
Illinois at Chicago, Chicago, IL 60612.
The Synthesis and Use of Stable Isotope-Labeled Peptide Substrates in Mechanistic Studies of Oligosaccharyl-transferase, Tong Xu and James K. Coward, Department of Chemistry and Interdepartmental Program of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109-1055.
Hydroxyl Radical Induced Hydrogen–Deuterium Exchange in Amino Acid Carbon–Hydrogen Bonds, Micheal B. Goshe and Vernon Anderson, Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106-4935.
The pH Dependence on the Reaction Catalyzed by Avian Phosphoenolpyruvate Carboxykinase, T. Holyoak and T. Nowak, Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556.
Stereospecific Synthesis of Thiol Analogs of Phosphatidylinositol, Cornelia Mihai, Jan Mataka, and Karol S. Bruzik, Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, Chicago, IL 60612.
Cloning, Expression, and Site-Directed Mutagenesis of Methylglyoxal Synthase from Escherichia coli, Dana Saadat and David H.T. Harrison, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226.
Studies toward the Synthesis of Chitobiose C-Glycoside Derivatives as Inhibitors of Oligosaccharyltransferase, Xinggao Fang and James K. Coward, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109.
Extreme Stereospecificity of Fumarase: Mechanistic Implications, Kerry L. Fillgrove and Vernon E. Anderson, Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106-4935.
The pH Dependence of the Reaction Catalyzed by Yeast MG-Enolase, Dmitriy A. Vinarov and Thomas Nowak, Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556.
Origins of pH Dependence of Coenzyme Binding by Liver Alcohol Dehydrogenase, Laurie A. LeBrun, Doo-Hong Park, and Bryce V. Plapp, Department of Biochemistry, University of Iowa, Iowa City, IA 52242.
The General Base in UDP-Galactose 4-Epimerase Is Revealed as Denaturation–Renaturation–Uncovers Charge–Transfer Complex, Yijeng Liu, Andrew M. Gulick, James B. Thoden, and Perry A. Frey, Institute for Enzyme Research, University of Wisconsin-Madison, Madison, WI 53705.
New and Improved Inactivators of Protein Tyrosine Phosphatases, William P. Taylor, Juliatiek Roestamadji, and Theordore S. Widlanski, Department of Chemistry, Indiana University, Bloomington, IN 47405.
Dissociative Inhibition of Restriction Endonucleases Eco RI and Bam HI By Synthetic -Helical Peptides, Michelle L. Bricker and Jean Chmielewski, Department of Chemistry, Purdue University, West Lafayette, IN 47907-1393.
The Role of Lysine 240 in Yeast Pyruvate Kinase, Thomas J. Bollenbach and Thomas Nowak, Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556.
Identification of the Active Site Residues of ACC Oxidase Involved in Metal Binding, Douglass C. Fahnone, Hamzah M. Salleh, Mayur A. Patel, Mahmoud A. Tayeh, and Ronald W. Woodard, Interdepartmental Program in Medicinal Chemistry, University of Michigan, Ann Arbor, MI 48109-1065.
Enzyme Electrostatics: Evaluation and Analysis of pH-Dependent Processes, Boris P. Atanasov, Institute of Organic Chemistry at Bulgarian Academy of Sciences, 1113-Sofia, Bulgaria; and Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637.
Endothiopeptide Inhibitors of HIV-1 Protease, Shao Yao, Reena Zutshi, and Jean Chmielewski, Department of Chemistry, Purdue University, West Lafayette, IN 47907.
DNA Shuffling of TEM-1 -Lactamase, Ichiro Matsumura and Andrew D. Ellington, Department of Chemistry, Indiana University, Bloomington, IN 47405.
Facile in Vitro Synthesis of ATP by Calcium Phosphates, W. Terry Jenkins, Department of Chemistry, Indiana University, Bloomington, IN 47401.
Dimerization Inhibitors of HIV-1 Protease, Michael D. Shultz, Reena Zutshi, Barbara Schweitzer, and Jean A. Chmielewski, Department of Chemistry, Purdue University, West Lafayette, IN 47907.
Radioactively Labeled Mitochondrial Monoamine Oxidase and Its Peptide Map, Boyu Zhong, Richard B. Silverman, and Paul A. Zieske, Departments of Chemistry and Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208.
Biosynthetic Pathway for Methylcinnamate in Basil, Zhigang Hao, Denys J. Charles, and James E. Simon, Center for New Crops and Plants Products, Purdue University, West Lafayette, IN 47907–1165.
Characterization of Phosphoribulokinase–Nucleotide Binary Complexes: Evidence for the Structural Integrity of Catalytic Mutants, J. A. Runquist, H. A. Koteiche, and H. M. Miziorko, Medical College of Wisconsin, Milwaukee, WI 53226.
Electrical Field Effects on Ligand Trafficking in the Active Site Gorge of AchE, Siobhan Malany and Daniel M. Quinn, Department of Chemistry, University of Iowa, Iowa City, IA 52242.
A Biomimetic and Molecular Modeling Approach to Rat Pancreatic Cholesterol Esterase Lipid Interfacial Recognition, Shawn R. Feaster, David Y. Hui, Greg Retzinger, and Daniel M. Quinn, Department of Chemistry, University of Iowa, Iowa City, IA 52242; and Department of Pathology, University of Cincinnati, Cincinnati, OH 45267.
Theoretical Studies of Serine Enzymes, Nathan A. Baker and Daniel M. Quinn, Department of Chemistry, University of Iowa, Iowa City, IA 52245.
The Evaluation of Potential Substrates for or Inhibitors of Mammalian Protein-Farnesyl Transferase, Richard Gibbs, Todd Zahn, Yong Qi Mu, Barbara Gibbs, and Judith Seybolt-Leopold, Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI 48202; and Department of Cell Biology, Parke–Davis, Ann Arbor, MI 48105.
Enoyl-CoA Hydratase: An Improved Purification Method to Maximize Purity and Yield, Jennifer E. Grant and Vernon E. Anderson, School of Medicine, Case Western Reserve University, Cleveland, OH 44106-4935.